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MacVector Help

https://www.macvector.com

What's New in MacVector?
Learn about changes in MacVector since the previous release.

Support
Learn more about this help and where to get additional help if needed.

MacVector Overview
Introduction to MacVector

Bringing Sequences into MacVector
How to bring your sequences into MacVector.

Using MacVector
Basic principles of MacVector.

Analyzing sequences
Restriction enzyme, alignments, primer design and subsequence searches.

Searching Databases
Accessing online databases, BLAST searches and local sequence searches.

Sequence Assembly
Contig Assembly, align cDNAs against genomic sequences and find SNPs.

Cloning

How do I check the orientation of a ligated fragment.

How do I subclone digested fragments into a vector

How do I design Gibson Assembly/LIC strategies.

How do I determine RE sites for cloning.

How do I show/hide the RE Picker tool?

Misc.

How do I import Genbank data from a web browser

How to do an online keyword search for sequences.

How do I bring sequences into MacVector.

How do I optimize codon usage.

How do I generate a transcript.

How do I translate a sequence.

Annotation

How do I automatically annotate blank sequences or change the style of a sequence map.

How do I display missing features, predicted ORFs and restriction sites on my sequence.

How do I annotate a gene feature to my sequence.

How do I change the default appearance of features.

How do I import Features from a Genome Browser.

How do I determine RE sites for cloning.

Primer design

How do I add a primer to the Primer Database

How do I design primers with tails and mismatches.

How do I design a pair of primers to amplify a gene?

How do I design a primer to match an existing primer

How do I make a permanent note of where my primer binds on a sequence

How do I test a pair of primers?

Assembly

How do I quickly align sequencing reads against a reference sequence.

How Do I Map NGS data against a reference genome

How do I create a de novo assembly using Velvet

How do I create a de novo assembly using SPAdes

How do I assemble PacBio and NanoPore reads using Flye

How Do I start an Assembly Project

How do I finish a genome assembly

How do I extract specific reads from a FASTQ dataset.

How do I do compare read coverage of different reference assemblies?

How do I automatically sort a sequencing dataset into subprojects

Alignments

How Do I identify important differences between two genomes

How Do I visually align a pair of sequences.

How do I do an online BLAST search.

How Do I choose the right alignment tool.